Oxidized sodium alginate used in an extracorporeal circulation pipe

doi:10.3969/j.issn.2095-4344.2013.34.015

Abstract

Abstract:

BACKGROUND: At present, a heparin-coated extracorporeal circulation pipe is used widely, but the price is expensive and limits its wide application.

OBJECTIVE: To screen the optimal coating condition of oxidated sodium alginate as a coating material to coat the medical polyvinyl chloride pipe and to evaluate the anticoagulant properties and the stability of the coated pipe.

METHODS: Oxidized sodium alginate was prepared with sodium periodate, and the mole ratio of sodium periodate and sodium alginate was 1:8, 1:10 and 1:12, respectively. Oxidized sodium alginate with different degree of oxidation was coated on the surface of medical polyvinyl chloride pipes by chemical methods, then to select the best degree of oxidation. The optimal coating condition was screened through an orthogonal experiment based on concentrations of sulfuric acid, polyethyleneimine and oxidized sodium alginate, and pH value and temperature of oxidized sodium alginate. Additionally, the anticoagulant properties and expulsion rates of the prepared pipes were evaluated in comparison with the blank control group and heparin-coated group.

RESULTS AND CONCLUSION: The optimal oxidation degree for oxidized sodium alginate was 1:10 for the mole ratio of sodium periodate and sodium alginate. The best coating conditions were 50% concentrated sulfuric acid, 0.05% polyethyleneimine, 40 ℃ of reaction temperature, 2 g/L oxidized sodium alginate, and pH value=3.5. The oxidized sodium alginate coating group had a similar trend compared with heparin-coated group in the expulsion rate. The anticoagulant properties of oxidized sodium alginate coating group were little weaker than those of heparin-coated group (P < 0.05), but significantly better than those of the blank control group. The oxidized sodium alginate-coated extracorporeal circulation pipe has a good anticoagulant property and stability.

Key words: biomaterials, biomaterials and drug controlled release, extracorporeal circulation, sodium alginate, polyvinyl chloride, coating, anticoagulant properties, stability, degree of oxidation, blood compatibility, provincial grants-supported paper

CLC Number:

R318

Li Jin-you, Li Tong, Yu Mei-li, Gao Wen-qing, Hu Xiao-min, Lu Hai-bin, Yu Guang-dong. Oxidized sodium alginate used in an extracorporeal circulation pipe[J]. Chinese Journal of Tissue Engineering Research, 2013, 17(34): 6159-6165.

Figures/Tables 8

References

[1]Thiara AS,Andersen VY,Videm V,et al. Comparable biocompatibility of Phisio- and Bioline-coated cardiopulmonary bypass circuits indicated by the inflammatory response. Perfusion.2010;25(1):9-16.

[2]Gunaydin S,Farsak B,McCusker K, et al. Clinical and biomaterial evaluation of hyaluronan-based heparin-bonded extracorporeal circuits with reduced versus full systemic anticoagulation in reoperation for coronary revascularization. Cardiovasc Med (Hagerstown).2009;10(2):135-142.

[3]Luo P,Liu W,Yang C,et al. Preparation and antithrombogenicity of oxidated low molecular weight heparin- antithrombin complex coated-polyvinyl chloride tubing.Sheng Wu Yi Xue Gong Cheng Xue Za Zhi.2011;28(1):90-94.

[4]Larm O,Larsson R,Olsson P.A new non-thrombogenic surface prepared by selective covalent binding of heparin via a modified reducing terminal residue. Biomater Med Devices Artif Organs.1983;11(2-3):161-234.

[5]Nozohoor S,Johnsson P,Scicluna S,et al. A case-controlled evaluation of the Medtronic Resting Heart System compared with conventional cardiopulmonary bypass in patients undergoing isolated coronary artery bypass surgery. Interact Cardiovasc Thorac Surg.2012;14(5):599-604.

[6]Mahmood S,Bilal H,Zaman M,et al.Is a fully heparin-bonded cardiopulmonary bypass circuit superior to a standard cardiopulmonary bypass circuit? Interact Cardiovasc Thorac Surg.2012;14(4):406-414.

[7]Moreno I,Soria A,López Gómez A,et al.Extracorporeal membrane oxygenation after cardiac surgery in 12 patients with cardiogenic shock. Rev Esp Anestesiol Reanim.2011;58(3):156-160.

[8]Hsu KH,Chi NH,Yu HY,et al. Extracorporeal membranous oxygenation support for acute fulminant myocarditis:analysis of a single center's experience. Cardiothorac Surg.2011；40(3): 682-688.

[9]Riscili BP,Anderson TB,Prescott HC,et al.An assessment of H1N1 influenza-associated acute respiratory distress syndrome severity after adjustment for treatment characteristics. PLoS One.2011; 6(3): e18166.

[10]Nishino T,Yokoyama G,Dobashi K,et al.Isolation, purification, and characterization of fucose-containing sulfated polysaccharides from the brown seaweed Ecklonia kurome and their blood-anticoagulant activities. Carbohydr Res.1989；186(1):119-148.

[11]Arora S,Budhiraja RD. Chitosan-alginate microcapsules of amoxicillin for gastric stability and mucoadhesion. Adv Pharm Technol Res. 2012;3(1):68-74.

[12]Del Gaudio P,Russo P,Rosaria Lauro M,et al. Encapsulation of ketoprofen and ketoprofen lysinate by prilling for controlled drug release.AAPS PharmSciTech.2009;10(4):1178-1185.

[13]Barnett BP,Arepally A,Stuber M,et al.Synthesis of magnetic resonance-,X-ray-and ultrasound-visible alginate microcapsules for immunoisolation and noninvasive imaging of cellular therapeutics.Nat Protoc.2011;6(8):1142-1151.

[14]Park SJ,Shin S,Koo OJ,et al.Functional improvement of porcine neonatal pancreatic cell clusters via conformal encapsulation using an air-driven encapsulator.Exp Mol Med. 2012;1(44):20-25.

[15]Shao S,Gao Y,Xie B,et al.Correction of hyperglycemia in type 1 diabetic models by transplantation of encapsulated insulin-producing cells derived from mouse embryo progenitor. J Endocrinol. 2011(208):245-255.

[16]高长路,刘颖新,武林枫.微囊化甲状旁腺细胞移植的研究进展[J]. 黑龙江医学, 2009,2(2):115-119.

[17]Sapir Y,Cohen S,Friedman G,et al.The promotion of in vitro vessel-like organization of endothelial cells in magnetically responsive alginate scaffolds. Biomaterials.2011;33(16): 4100-4109.

[18]Xia Y,Mei F,Duan Y,et al. Bone tissue engineering using bone marrow stromal cells and an injectable sodium alginate/gelatin scaffold.Biomed Mater Res A.2012;100(4): 1044-1050.

[19]Weir MD,Xu HH.Human bone marrow stem cell-encapsulating calcium phosphate scaffolds for bone repair.Acta Biomater.2010;10(6):4118-4126.

[20]Forster RE,Thürmer F,Wallrapp C, et al.Characterisation of physico-mechanical properties and degradation potential of calcium alginate beads for use in embolisation . Mater Sci Mater Med.2010;5(21):2243-2251.

[21]Bouhadir KH,Lee KY,Alsberg E,et al.Degradation of partially oxidized alginate and its potential application for tissue engineering. Biotechnol Prog.2001;17(5):945-995.

[22]何淑兰,张敏,耿占杰,等.部分氧化海藻酸钠的制备与性能[J].应用化学,2005,22(9):1007-1011.

[23]钟方晓,任海华,李岩.多糖含量测定方法比较[J].时珍国医国药, 2007,18(8):1916-1917.

[24]Gao C,Liu M,Chen J,et al.Interactions between bovine serum albumin and oxidized sodium alginate in solution.Biomater Sci Polym Ed. 2011;22(12):1639-1650.

[25]梁晔,刘万顺,韩宝芹,等.一种新型生物交联剂的制备及其性质[J].中国海洋大学学报,2008,38(4) : 590-594.

[26]Andersson LO,Hoffman J,Holmer E,et al.Mechanisms of anticoagulant effects of some sulphated polysaccharides. Thromb Res.1982;28(6):741-748.

[27]Mirow N,Zittermann A,Koertke H,et al.Heparin-coated extracorporeal circulation in combination with low dose systemic heparinization reduces early postoperative blood loss in cardiac surgery.Cardiovasc Surg (Torino). 2008;49(2): 277-284.

[28]Ovrum E,Tangen G,Tølløfsrud S,et al. Heparinized cardiopulmonary bypass circuits and low systemic anticoagulation: an analysis of nearly 6000 patients undergoing coronary artery bypass grafting. Thorac Cardiovasc Surg.2011;141(5):1145-1149.